In early stages of biotherapeutic development, generic HCP ELISA’s are an easy-to-use solution to quantify HCP content in DS and show HCP clearance through the DSP. However, reliable HCP quantification can be biased by the use of non-validated generic ELISAs. If generic ELISAs are used, validation of their performance including … Continue reading →

Controlling Residual Host Cell Proteins (HCP) Impurities in Biopharmaceuticals Biotherapeutics must be free of Bioprocess-related impurities, including protein contaminants derived from the Host Cell used as the expression system (bacterium, yeast, mammalian, insect or plant cells). HCP fit the definition of the Critical Quality Attribute (CQA) due to their potential … Continue reading →

Digitization, Interpretation and Archiving of the results obtained with the IDRing® plates (SRID Tests) Single Radial ImmunoDiffusion (SRID) is very easy to use analytical method. It is used in many industries (pharmaceutical, veterinary, food processing, biotechnology) to quantify target proteins and to perform batch releases. In order to comply with … Continue reading →

Evaluation of bovine colostrum powder quality by quantitation of native IgG : why to choose the Single Radial ImmunoDiffusion (SRID) Assay ?   The importance of colostrum to the health and growth of newborn mammals is well known. In the bovine, the maternal immunity is quite exclusively transferred to the … Continue reading →

Allergy to goat and sheep milk without allergy to cows’ milk Cows’ milk allergy occurs in 2% to 6% of the infant population, being the most frequent cause of food allergy. Many of these infants cannot tolerate goats’ or sheeps’ milk either (Bellioni-Businjco et al, 1999). Conversely, the goat’s or … Continue reading →

Alkaline Phophatase activity (ALP) in milk and cheese   Alkaline phosphatase is a naturally occurring enzyme in milk. Because thermal resistance of the enzyme is greater than that of non-spore forming pathogenic microorganisms commonly found in milk, the detection of alkaline phosphatase is used as a legal test to determine … Continue reading →

Inactivation of alkaline phosphatase as a control of colostrum pasteurization: are you sure to monitor the milk alkaline phosphatase? First-milking colostrum is an important source of growth factors, vitamins, minerals and maternal antibodies, critical to protect the newborn against infectious diseases in the first weeks and months of life. In … Continue reading →